CD55 Recombinant antibody

CD55 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2

Cat no : 82781-6-RR

Synonyms

TC, DAF, CROM, CR, Complement decay-accelerating factor

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Validation Data Gallery

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  WB analysis using 82781-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82781-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 82781-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82781-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82781-6-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 82781-6-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82781-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82781-6-RR against Human CD55 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, MKN-45 cells, human placenta tissue, K-562 cells
• Positive IHC detected in: human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82781-6-RR targets CD55 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD55 fusion protein Ag25026
• Full Name: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001288
• Gene Symbol: CD55
• Gene ID (NCBI): 1604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).