CD55 Recombinant antibody, PBS Only

CD55 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2

Cat no : 82781-6-PBS

Synonyms

TC, DAF, CROM, CR, Complement decay-accelerating factor

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Validation Data Gallery

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  WB analysis using 82781-6-RR (same clone as 82781-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 82781-6-RR (same clone as 82781-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82781-6-RR (same clone as 82781-6-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD55 antibody (82781-6-RR, Clone: 2D2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82781-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82781-6-RR against Human CD55 were performed. The affinity constant is below 1 pM.

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Product Information

82781-6-PBS targets CD55 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD55 fusion protein Ag25026
• Full Name: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001288
• Gene Symbol: CD55
• Gene ID (NCBI): 1604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).