CoraLite® Plus 555 Anti-Human ICAM-1/CD54 (15.2)
ICAM-1/CD54 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 555 Fluorescent Dye
CloneNo.
15.2
Cat no : CL555-65075
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Rheumatoid synovial cells and human monocytes |
Full Name | intercellular adhesion molecule 1 |
Calculated Molecular Weight | 90 kDa |
GenBank Accession Number | BC015969 |
Gene Symbol | ICAM-1 |
Gene ID (NCBI) | 3383 |
Conjugate | CoraLite® Plus 555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 555 ICAM-1/CD54 antibody CL555-65075 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |