CoraLite® Plus 647 Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombinant Antibody

ICAM-1/CD54 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

15.2

Cat no : CL647-65567

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using CL647-65567

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD3, and 5 ul Coralite® Plus 647 Cd54 Recombinant Antibody (CL647-65567, Clone:15.2) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65567

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD3 and 5 ul Coralite® Plus 647 Cd54 Recombinant Antibody (CL647-65567, Clone:15.2) (right) or 5 ul CoraLite® Plus 647 Anti-Human CD54 (ICAM-1) (15.2) (CL647-65075, Clone: 15.2) (left). Cells were not fixed. Lymphocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65567 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).