CoraLite® Plus 647 Anti-Human CD5 (L17F12) Mouse IgG1 Recombinant Antibody

CD5 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

L17F12

Cat no : CL647-65530

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65530

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD5 Mouse Recombinant Antibody (CL647-65530, Clone:L17F12) or CoraLite® Plus 647 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65530

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD5 Mouse Recombinant Antibody (CL647-65530, Clone:L17F12) or CoraLite® Plus 647 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65530

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD5 Mouse Recombinant Antibody (CL647-65530, Clone:L17F12) or 5 ul CoraLite® Plus 647 Anti-Human CD5 Mouse Monoclonal Antibody (CL647-65200, Clone:L17F12). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65530 targets CD5 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia (T-ALL) cells
• Full Name: CD5 molecule
• Calculated Molecular Weight: 495 aa, 55 kDa
• GenBank Accession Number: BC027901
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).