1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2), or unstained cells. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2), or unstained cells. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using Biotin-65609
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2) (red), or unstained cells (blue). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2) (red), or unstained cells (blue). Cells were not fixed. Lymphocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
Biotin-65609 targets CD5 in FC applications and shows reactivity with human samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2), or unstained cells. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using Biotin-65609
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2) (red), or unstained cells (blue). Cells were not fixed. Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD5 Recombinant antibody
Catalog Number
Biotin-65609
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
