CD5 Monoclonal antibody, PBS Only

CD5 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D12C10

Cat no : 67278-1-PBS

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

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Validation Data Gallery

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  IHC staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

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Product Information

67278-1-PBS targets CD5 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD5 fusion protein Ag11065
• Full Name: CD5 molecule
• Calculated Molecular Weight: 495 aa, 55 kDa
• GenBank Accession Number: BC027901
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).