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CD5 Monoclonal antibody

CD5 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4D12C10

Cat no : 67278-1-Ig

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IHC staining of human tonsillitis using 67278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IHC staining of human tonsillitis using 67278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IHC staining of human tonsillitis using 67278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IF Staining of human tonsillitis using 67278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IF Staining of human tonsillitis using 67278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67278-1-Ig targets CD5 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD5 fusion protein Ag11065
Full Name CD5 molecule
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
RRIDAB_2882546
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
IHC protocol for CD5 antibody 67278-1-IgDownload protocol
IF protocol for CD5 antibody 67278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols