APC Anti-Mouse CD49b (DX5)
CD49b Monoclonal Antibody for FC
Host / Isotype
Rat / IgM, kappa
Reactivity
Mouse
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
DX5
Cat no : APC-65065
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | mouse splenocytes |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 1 publications below |
Product Information
APC-65065 targets CD49b in FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Host / Isotype | Rat / IgM, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | NK cells isolated from C57BL/6 mice |
Full Name | integrin alpha 2 |
GenBank Accession Number | BC065139 |
Gene Symbol | CD49b |
Gene ID (NCBI) | 16398 |
RRID | AB_2882967 |
Conjugate | APC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and a stabilizer. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.
Protocols
Product Specific Protocols | |
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FC protocol for APC CD49b antibody APC-65065 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Liver Int Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury |