Anti-Human CD47 Rabbit Recombinant Antibody, PBS Only

CD47 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240997D8

Cat no : 98131-1-PBS

Synonyms

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using 98131-1-RR (same clone as 98131-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD47 Rabbit Recombinant Antibody (98131-1-RR, Clone: 240997D8) or 0.25 ug Isotype Control, and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98131-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98131-1-RR (same clone as 98131-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD47 Rabbit Recombinant Antibody (98131-1-RR, Clone:240997D8) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98131-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98131-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98131-1-RR against Human CD47 were performed. The affinity constant is 0.723 nM.

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Product Information

The immunogen of 98131-1-PBS is CD47 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD47 molecule
• Calculated Molecular Weight: 323 aa, 35 kDa
• GenBank Accession Number: BC010016
• Gene Symbol: CD47
• Gene ID (NCBI): 961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.