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Anti-Human CD47 Rabbit Recombinant Antibody

CD47 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240997D8

Cat no : 98131-1-RR

Synonyms

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD47 Rabbit Recombinant Antibody (98131-1-RR)

    FC experiment of human PBMCs using 98131-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD47 Rabbit Recombinant Antibody (98131-1-RR, Clone: 240997D8) or 0.25 ug Isotype Control, and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD47 Rabbit Recombinant Antibody (98131-1-RR)

    FC experiment of human PBMCs using 98131-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD47 Rabbit Recombinant Antibody (98131-1-RR, Clone:240997D8) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98131-1-RR

    Affinity and Kinetic Characterization of 98131-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98131-1-RR against Human CD47 were performed. The affinity constant is 0.723 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98131-1-RR is CD47 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD47 molecule
Calculated Molecular Weight 323 aa, 35 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Protocols

Product Specific Protocols
FC protocol for CD47 antibody 98131-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols