CoraLite® Plus 647 Anti-Rat CD45RA (OX-33)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX-33

Cat no : CL647-65234

Synonyms

CD45, CD45RA, L CA, Lca, Ptprc, RT7, T200

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Validation Data Gallery

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  FC experiment of rat splenocytes using CL647-65234

  1X10^6 rat splenocytes cells were surface co-stained with FITC Anti-Rat CD4 (OX-35) and 0.5 ug CoraLite® Plus 647 Anti-Rat CD45RA (CL647-65234, Clone: OX-33) or 0.5 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21). Cells were not fixed.

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Tested Applications

• Positive FC detected in: rat splenocytes cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65234 targets CD45RA in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: CD45RA
• Gene ID (NCBI): 24699
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).