CoraLite® Plus 647 Anti-Rat CD45RA (OX-33)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX-33

Cat no : CL647-65234

Synonyms

CD45, CD45RA, L CA, Lca, Ptprc, RT7, T200



Tested Applications

Positive FC detected inrat splenocytes cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65234 targets CD45RA in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Leukocyte common antigen purified from rat splenocytes
Full Name protein tyrosine phosphatase, receptor type, C
Calculated Molecular Weight143 kDa
GenBank Accession NumberXM_006249910
Gene Symbol CD45RA
Gene ID (NCBI) 24699
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD45RA antibody CL647-65234Download protocol
Standard Protocols
Click here to view our Standard Protocols