PerCP Anti-Human CD45 (HI30)
CD45 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
PerCP Fluorescent Dye
CloneNo.
HI30
Cat no : PerCP-65109
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
PerCP-65109 targets CD45 in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Human peripheral blood leucocytes |
Full Name | protein tyrosine phosphatase, receptor type, C |
GenBank Accession Number | BC014239 |
Gene Symbol | PTPRC |
Gene ID (NCBI) | 5788 |
Conjugate | PerCP Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 482 nm / 677 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
Protocols
Product Specific Protocols | |
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FC protocol for PerCP CD45 antibody PerCP-65109 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |