CoraLite® Plus 647 Anti-Human CD45 (HI30)

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  IF Staining of Jurkat using CL647-65109

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 647 CD45 antibody (CL647-65109, Clone: HI30 ) at dilution of 1:200.

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  FC experiment of human PBMCs using CL647-65109

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD45 (CL647-65109, Clone: HI30), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD45 Monoclonal Antibody for IF/ICC, FC

Cat No. CL647-65109

Clone No.HI30

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Synonyms

EC:3.1.3.48, GP180, HI30, L CA, LCA

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65109 targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human peripheral blood leucocytes

• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_2883687
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08575
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).