CoraLite® Plus 488-conjugated CD45 Recombinant antibody

CD45 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240356G10

Cat no : CL488-83396-5

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 240356G10

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Validation Data Gallery

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  IF Staining of RAW 264.7 using CL488-83396-5

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 CD45 antibody (CL488-83396-5, Clone: 240356G10 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83396-5 is CD45 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 145 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_001111316.2
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).