CoraLite® Plus 488-conjugated CD45 Recombinant antibody

CD45 Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6O19

Cat no : CL488-80297

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  IF Staining of mouse spleen using CL488-80297

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 CD45 antibody (CL488-80297, Clone: 6O19 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using CL488-80297

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 CD45 antibody (CL488-80297, Clone: 6O19 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF detected in: mouse spleen tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80297 targets CD45 in IF applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD45 fusion protein Ag13712
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 1304 aa, 147 kDa
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC014239
• Gene Symbol: PTPRC
• Gene ID (NCBI): 5788
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.