Anti-Human CD45 (HI30), PBS Only

• 

  IF Staining of Jurkat using 65109-1-Ig (same clone as 65109-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65109-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65109-1-Ig (same clone as 65109-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45 (HI30) (65109-1-Ig, Clone: HI30) or Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65109-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65109-1-Ig (same clone as 65109-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45 (HI30) (65109-1-Ig, Clone: HI30) (red) or Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65109-1-PBS in a different storage buffer formulation.

CD45 Monoclonal Antibody for IF/ICC, FC

Cat No. 65109-1-PBS

Clone No.HI30

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Synonyms

EC:3.1.3.48, GP180, HI30, L CA, LCA

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Product Information

65109-1-PBS targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human peripheral blood leucocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_2918407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08575
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).