CD45(220-577) Recombinant antibody, PBS Only (Capture)

CD45(220-577) Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241671G12

Cat no : 84405-4-PBS

Synonyms

CD45, L CA, GP180, EC:3.1.3.48, B220

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01280-3

  Sandwich ELISA standard curve of MP01280-3, Human CD45 Recombinant Matched Antibody Pair - PBS only. 84405-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2486. 84405-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

• 

  Sample test of MP01280-3

  Plasma of six individual healthy human donors was measured. The CD45 concentration of detected samples was determined to be 8,737.3 pg/mL with a range of 4,942.4 -15,153.0 pg/mL.

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  Sample test of MP01280-3

  The mean CD45 concentration was determined to be 87.3 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP01280-1

  Cytometric bead array standard curve of MP01280-1, CD45 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84405-4-PBS. Detection antibody: 84405-3-PBS. Standard: Eg2486. Range: 0.625-80 ng/mL.

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Product Information

84405-4-PBS targets CD45(220-577) as part of a matched antibody pair:

MP01280-1: 84405-4-PBS capture and 84405-3-PBS detection (validated in Cytometric bead array)

MP01280-3: 84405-4-PBS capture and 84405-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.