CoraLite® Plus 647 Anti-Mouse CD45.1 (A20)

CD45.1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

A20

Cat no : CL647-65092

Synonyms

A20, B220, CD45.1, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200



Tested Applications

Positive FC detected inSJL mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65092 targets CD45.1 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen SJL mouse thymocytes and splenocytes
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol CD45R
Gene ID (NCBI) 19264
RRIDAB_2883676
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD45.1 antibody CL647-65092Download protocol
Standard Protocols
Click here to view our Standard Protocols