Anti-Human CD44 (F10-44-2) Rabbit Recombinant Antibody

CD44 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

F10-44-2

Cat no : 65502-1-RR

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Validation Data Gallery

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  FC experiment of human PBMCs using 65502-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-RR, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Titrations: 0.4, 0.2, 0.1, 0.05ug

• 

  FC experiment of human PBMCs using 65502-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-RR, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65502-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-PBS, Clone:F10-44-2) (red) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD44 Mouse Monoclonal Antibody (Clone:F10-44-2) (65063-1-Ig in green; or from another vendor in pink) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65502-1-RR targets CD44 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Purified T cells from human lymph nodes
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 742 aa, 82 kDa
• GenBank Accession Number: BC004372
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.