Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 65608-1-MR (same clone as 65608-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Mouse IgG2a Recombinant Antibody (65608-1-MR, Clone: F10-44-2) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65608-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65608-1-MR (same clone as 65608-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Mouse IgG2a Recombinant Antibody (65608-1-MR, Clone: F10-44-2) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65608-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65608-1-MR (same clone as 65608-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Mouse IgG2a Recombinant Antibody (65608-1-MR, Clone:F10-44-2) (left) or 0.25 ug Anti-Human CD44 (65063-1-Ig, Clone: F10-44-2) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65608-1-PBS in a different storage buffer formulation.

CD44 Recombinant Antibody for FC

Cat No. 65608-1-PBS

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

65608-1-PBS targets CD44 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 742 aa, 82 kDa
• GenBank Accession Number: BC004372
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• ENSEMBL Gene ID: ENSG00000026508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.