CoraLite® Plus 750 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody

CD44 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

F10-44-2

Cat no : CL750-65608

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

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Validation Data Gallery

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  FC experiment of human PBMCs using CL750-65608

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite® Plus 750 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody (CL750-65608, Clone: F10-44-2) (right), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65608

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody (CL750-65608, Clone: F10-44-2) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65608 targets CD44 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 742 aa, 82 kDa
• GenBank Accession Number: BC004372
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.