CD43 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66224-1-Ig (same clone as 66224-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66224-1-Ig (CD43 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

CD43 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 66224-1-PBS

Clone No.2A11D6

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein, CD43 cytoplasmic tail

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Product Information

66224-1-PBS targets CD43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD43 fusion protein Ag23595
• Full Name: sialophorin
• Calculated Molecular Weight: 400 aa, 43 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC012350
• Gene Symbol: CD43
• Gene ID (NCBI): 6693
• ENSEMBL Gene ID: ENSG00000197471
• RRID: AB_2881615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16150
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.