CoraLite® Plus 594 Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC
Cat No. CL594-65163
Clone No.AK2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65163 targets CD42b in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human platelets Predict reactive species
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated Molecular Weight 626 aa, 69 kDa
GenBank Accession NumberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP07359
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Product Specific Protocols
FC protocol for CL Plus 594 CD42b antibody CL594-65163Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...