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CD40L/CD154 Monoclonal antibody, PBS Only

CD40L/CD154 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E6D10

Cat no : 66502-1-PBS

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40L/CD154, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD40L/CD154 Monoclonal antibody (66502-1-Ig)

    WB analysis of Jurkat using 66502-1-Ig (same clone as 66502-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66502-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using CD40L/CD154 Monoclonal antibody (66502-1-Ig)

    WB analysis of human peripheral blood platelets using 66502-1-Ig (same clone as 66502-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66502-1-PBS in a different storage buffer formulation.

Product Information

66502-1-PBS targets CD40L/CD154 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD40L/CD154 fusion protein Ag24941
Full Name CD40 ligand
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC071754
Gene Symbol CD40 Ligand
Gene ID (NCBI) 959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.