CD40L/CD154 Monoclonal antibody

CD40L/CD154 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1E6D10

Cat no : 66502-1-Ig

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40L/CD154, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 66502-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 66502-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

66502-1-Ig targets CD40L/CD154 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD40L/CD154 fusion protein Ag24941
• Full Name: CD40 ligand
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC071754
• Gene Symbol: CD40 Ligand
• Gene ID (NCBI): 959
• RRID: AB_2881866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

Publications

Species	Application	Title
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
human	IHC	Pathol Res Pract; The potential role of follicular helper T cells and helper T cells type 1 in Warthin tumour.; Authors - Yoshiaki Kobayashi
human	IHC	Pathol Res Pract; The potential role of follicular helper T cells in idiopathic multicentric Castleman disease with and without TAFRO syndrome.; Authors - Nozomu Kurose
human	IF	Br J Cancer; Integrated proteomics and scRNA-seq analyses of ovarian cancer reveal molecular subtype-associated cell landscapes and immunotherapy targets; Authors - Rong Tan