CD40 Recombinant antibody, PBS Only (Detector)

CD40 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241760F4

Cat no : 84406-5-PBS

Synonyms

p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01294-3

  Sandwich ELISA standard curve of MP01294-3, Human CD40 Recombinant Matched Antibody Pair - PBS only. 84406-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1797. 84406-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01294-3

  Saliva of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 829.1 pg/mL with a range of 270.5-2,164.5 pg/mL.

• 

  Sample test of MP01294-3

  Human milk of five individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 2,099.7 pg/mL with a range of 1,212.9-3,125.0 pg/mL.

• 

  Sample test of MP01294-3

  Urine of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 1,6246.6 pg/mL with a range of 5,532.8-21,569.7 pg/mL.

• 

  Sample test of MP01294-3

  MDA-MB-453 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CD40, and measured 76.3 pg/mL.

• 

  Sample test of MP01294-3

  Serum of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 2,121.2 pg/mL with a range of 1,366.2-2,760.0 pg/mL.

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Product Information

84406-5-PBS targets CD40 as part of a matched antibody pair:

MP01294-3: 84406-3-PBS capture and 84406-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: CD40 molecule, TNF receptor superfamily member 5
• Calculated Molecular Weight: 31kDa
• GenBank Accession Number: NM_001250.6
• Gene Symbol: CD40
• Gene ID (NCBI): 958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.