Anti-Human CD40 (G28.5), PBS Only

CD40 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

G28.5

Cat no : 65103-1-PBS

Synonyms

p50, G28.5, CD40L receptor, Bp50, B-cell surface antigen CD40

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50064-1

  Sandwich ELISA standard curve of MP50064-1, CD40 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66965-2-PBS. Detection antibody: 65103-1-PBS. Standard: Ag28040. Range: 78.1-5000 pg/mL.

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  FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

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Product Information

65103-1-PBS targets CD40 as part of a matched antibody pair:

MP50064-1: 66965-2-PBS capture and 65103-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD40 molecule, TNF receptor superfamily member 5
• Calculated Molecular Weight: 277 aa, 31 kDa
• GenBank Accession Number: BC012419
• Gene Symbol: CD40
• Gene ID (NCBI): 958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).