CoraLite® Plus 488-conjugated CD4 Recombinant antibody

CD4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240427E12

Cat no : CL488-83513-7

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

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Validation Data Gallery

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  IF Staining of THP-1 using CL488-83513-7

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 CD4 antibody (CL488-83513-7, Clone: 240427E12 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83513-7 is CD4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).