Anti-Human CD4 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 98042-1-RR (same clone as 98042-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98042-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98042-1-RR (same clone as 98042-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98042-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

CD4 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98042-1-PBS

Clone No.240427E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

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Product Information

98042-1-PBS targets CD4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01730
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).