CD4 Recombinant antibody, PBS Only (Capture)

CD4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240427E10

Cat no : 83513-3-PBS

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E10

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00503-2

  Sandwich ELISA standard curve of MP00503-2, CD4 Recombinant Matched Antibody Pair - PBS only. 83513-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0333.83513-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00503-2

  The mean CD4 concentration was determined to be 14.49 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

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  Sample test of MP00503-2

  Serum of eight individual healthy human donors was measured. The CD4 concentration of detected samples was determined to be 130 pg/mL with a range of 88.46 - 157.66 pg/mL.

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  Cytometric bead array standard curve of MP00503-2

  Cytometric bead array standard curve of MP00503-2, CD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83513-3-PBS. Detection antibody: 83513-4-PBS. Standard: Eg0333. Range: 0.313-40 ng/mL.

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Product Information

83513-3-PBS targets CD4 as part of a matched antibody pair:

MP00503-2: 83513-3-PBS capture and 83513-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.