CD3EAP Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84139-4-RR (same clone as 84139-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84139-4-RR (CD3EAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84139-4-RR (same clone as 84139-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CD3EAP Recombinant antibody (84139-4-RR, Clone:241379H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01056-3

  Cytometric bead array standard curve of MP01056-3, CD3EAP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84139-4-PBS. Detection antibody: 84139-2-PBS. Standard: Ag35074. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84139-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84139-4-RR against Human CD3EAP were performed. The affinity constant is 0.339 nM.

CD3EAP {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84139-4-PBS

Clone No.241379H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

CAST, ASE-1, ASE1, Antisense to ERCC-1 protein, A34.5

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Product Information

84139-4-PBS targets CD3EAP as part of a matched antibody pair:

MP01056-3: 84139-4-PBS capture and 84139-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD3EAP fusion protein Ag35074
• Full Name: CD3e molecule, epsilon associated protein
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC038992
• Gene Symbol: CD3EAP
• Gene ID (NCBI): 10849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15446
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.