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  • Western Blot (WB) analysis of various lysates using CD3EAP Recombinant antibody (84139-4-RR)

    WB analysis using 84139-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84139-4-RR (CD3EAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using CD3EAP Recombinant antibody (84139-4-RR)

    FC experiment of U2OS using 84139-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CD3EAP Recombinant antibody (84139-4-RR, Clone:241379H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84139-4-RR

    Affinity and Kinetic Characterization of 84139-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84139-4-RR against Human CD3EAP were performed. The affinity constant is 0.339 nM.

CD3EAP Recombinant antibody

CD3EAP {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84139-4-RR
Clone No.241379H5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CAST, ASE-1, ASE1, Antisense to ERCC-1 protein, A34.5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells, K-562 cells, HEK-293T cells, NCI-H1299 cells, HCT 116 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84139-4-RR targets CD3EAP in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD3EAP fusion protein Ag35074 Predict reactive species
Full Name CD3e molecule, epsilon associated protein
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight80-90 kDa
GenBank Accession NumberBC038992
Gene Symbol CD3EAP
Gene ID (NCBI) 10849
RRIDAB_3671699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15446
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.

Protocols

Product Specific Protocols
WB protocol for CD3EAP antibody 84139-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols