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CD3 Delta Recombinant antibody, PBS Only (Detector)

CD3 Delta Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241523H2

Cat no : 84214-4-PBS

Synonyms

CD3D, 241523H2, T-cell receptor T3 delta chain, T3D, T cell receptor T3 delta chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 Delta Recombinant antibody (84214-4-RR)

    WB analysis using 84214-4-RR (same clone as 84214-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84214-4-RR (CD3 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Delta Recombinant antibody (84214-4-RR)

    IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84214-4-RR (CD3 Delta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Delta Recombinant antibody (84214-4-RR)

    IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84214-4-RR (CD3 Delta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01149-2

    Cytometric bead array standard curve of MP01149-2

    Cytometric bead array standard curve of MP01149-2, CD3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84214-1-PBS. Detection antibody: 84214-4-PBS. Standard: Eg2288. Range: 0.625-80 ng/mL.

Product Information

84214-4-PBS targets CD3 Delta as part of a matched antibody pair:

MP01149-2: 84214-1-PBS capture and 84214-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD3d molecule, delta (CD3-TCR complex)
Calculated Molecular Weight 171 aa, 19 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC070321
Gene Symbol CD3 Delta
Gene ID (NCBI) 915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. CD3 delta/CD3D is a single-pass type I membrane protein which consists of an extracellular domain of 84 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. Defects in CD3 delta are a cause of severe combined immunodeficiency.