Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody, PBS Only

CD39/ENTPD1 Uni-rAb^TM Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241292G7

Cat no : 98102-1-PBS

Synonyms

ENTPD1, EC:3.6.1.5, CD39, ATP-DPH, ATPDase

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01143-1

  Sandwich ELISA standard curve of MP01143-3, Human CD39/ENTPD1 Recombinant Matched Antibody Pair - PBS only. 98102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02313. 84235-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP01143-1

  Plasma of six individual healthy human donors was measured. The CD39/ENTPD1 concentration of detected samples was determined to be 179.9 pg/mL with a range of 134.2-243.6 ng/mL.

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  Sample test of MP01143-1

  Serum of six individual healthy human donors was measured. The CD39/ENTPD1 concentration of detected samples was determined to be 373.5 pg/mL with a range of 283.0-450.4 pg/mL.

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  FC experiment of human PBMCs using 98102-1-RR (same clone as 98102-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR, Clone: 241292G7) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98102-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98102-1-RR (same clone as 98102-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR, Clone: 241292G7) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98102-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01143-1

  Cytometric bead array standard curve of MP01143-1, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98102-1-PBS. Detection antibody: 84235-2-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP01143-2

  Cytometric bead array standard curve of MP01143-2, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98102-1-PBS. Detection antibody: 84235-1-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

• 

  Cytometric bead array standard curve of MP01143-3

  Cytometric bead array standard curve of MP01143-3, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84235-3-PBS. Detection antibody: 98102-1-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

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Product Information

98102-1-PBS targets CD39/ENTPD1 as part of a matched antibody pair:

MP01143-1: 98102-1-PBS capture and 84235-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01143-2: 98102-1-PBS capture and 84235-1-PBS detection (validated in Cytometric bead array)

MP01143-3: 84235-3-PBS capture and 98102-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: ectonucleoside triphosphate diphosphohydrolase 1
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: BC047664
• Gene Symbol: CD39
• Gene ID (NCBI): 953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers (PMID: 9733785 ).