Biotin Anti-Human CD36 Rabbit Recombinant Antibody

CD36 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

241262D5

Cat no : Biotin-98132

Synonyms

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

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Validation Data Gallery

• 

  FC experiment of human PBMCs using Biotin-98132

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD36 Rabbit Recombinant Antibody (Biotin-98132, Clone: 241262D5) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human HLA-DR. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98132

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD36 Rabbit Recombinant Antibody (Biotin-98132, Clone: 241262D5) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of Biotin-98132 is CD36 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD36 molecule (thrombospondin receptor)
• Calculated Molecular Weight: 53 kDa
• GenBank Accession Number: NM_001001548.3
• Gene Symbol: CD36
• Gene ID (NCBI): 948
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

• Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

• Polymorphisms in CD36 can increase susceptibility to malaria.

• AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

• Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.