Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody, PBS Only

CD35 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

E11

Cat no : 65622-1-PBS

Synonyms

KN, E11, CR1, Complement receptor type 1, C3BR

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Validation Data Gallery

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  FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65622-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65622-1-PBS in a different storage buffer formulation.

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Product Information

65622-1-PBS targets CD35 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
• Full Name: complement component (3b/4b) receptor 1 (Knops blood group)
• Calculated Molecular Weight: 224 kDa
• GenBank Accession Number: NM_000573
• Gene Symbol: CR1
• Gene ID (NCBI): 1378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.