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Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody

CD35 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

E11

Cat no : 65622-1-MR

Synonyms

KN, E11, CR1, Complement receptor type 1, C3BR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD35 (E11) Mouse IgG2a Recombinant Anti (65622-1-MR)

    FC experiment of human PBMCs using 65622-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD35 (E11) Mouse IgG2a Recombinant Anti (65622-1-MR)

    FC experiment of human PBMCs using 65622-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65622-1-MR targets CD35 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated Molecular Weight 224 kDa
GenBank Accession NumberNM_000573
Gene Symbol CR1
Gene ID (NCBI) 1378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

Product Specific Protocols
FC protocol for CD35 antibody 65622-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols