CoraLite® Plus 488-conjugated CD34 Monoclonal antibody

CD34 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

3C8G12

Cat no : CL488-60180

Synonyms

CD34 molecule, 3C8G12

---

Validation Data Gallery

• 

  IF Staining of human tonsillitis using CL488-60180

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-60180 (CD34 antibody), at dilution of 1:100.

• 

  IF Staining of human placenta using CL488-60180

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 CD34 antibody (CL488-60180, Clone: 3C8G12 ) at dilution of 1:50. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IF-P detected in: human tonsillitis tissue, human placenta tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-60180 targets CD34 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD34 fusion protein Ag5996
• Full Name: CD34 molecule
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: BC039146
• Gene Symbol: CD34
• Gene ID (NCBI): 947
• RRID: AB_2883112
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Publications

Species	Application	Title
	IF	Immunopharmacol Immunotoxicol; Fraxetin protects rat brains from the cerebral stroke via promoting angiogenesis and activating PI3K/Akt pathway.; Authors - Yuhuan Cui