CD34 Recombinant antibody, PBS Only

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  WB analysis using 84242-5-RR (same clone as 84242-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84242-5-RR (same clone as 84242-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84242-5-RR (same clone as 84242-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84242-5-RR (same clone as 84242-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84242-5-RR (same clone as 84242-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84242-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

CD34 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84242-5-PBS

Clone No.242069F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CD34 molecule, 242069F8

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Product Information

84242-5-PBS targets CD34 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD34 molecule
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC039146
• Gene Symbol: CD34
• Gene ID (NCBI): 947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P28906
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.