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CD34 Recombinant antibody

CD34 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

242069F8

Cat no : 84242-5-RR

Synonyms

CD34 molecule, 242069F8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD34 Recombinant antibody (84242-5-RR)

    WB analysis using 84242-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human placenta using 84242-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human placenta using 84242-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84242-5-RR

    Affinity and Kinetic Characterization of 84242-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

Tested Applications

Positive WB detected inHL-60 cells, bEnd.3 cells, HUVEC cells, mouse testis tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84242-5-RR targets CD34 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD34 molecule
Calculated Molecular Weight 41 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
WB protocol for CD34 antibody 84242-5-RRDownload protocol
IHC protocol for CD34 antibody 84242-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols