Mouse CD34 Recombinant antibody, PBS Only (Capture)

CD34 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240659C7

Cat no : 83713-2-PBS

Synonyms

CD34 antigen, 240659C7

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Validation Data Gallery

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  WB analysis using 83713-2-RR (same clone as 83713-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83713-2-RR (CD34 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83713-2-RR (same clone as 83713-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83713-2-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 83713-2-RR (same clone as 83713-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD34 antibody (83713-2-RR, Clone: 240659C7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00684-1

  Cytometric bead array standard curve of MP00684-1, MOUSE CD34 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83713-2-PBS. Detection antibody: 83713-4-PBS. Standard: Eg0854. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83713-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83713-2-RR against Mouse CD34 were performed. The affinity constant is 1.09 nM.

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Product Information

83713-2-PBS targets CD34 as part of a matched antibody pair:

MP00684-1: 83713-2-PBS capture and 83713-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD34 antigen
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_133654
• Gene Symbol: CD34
• Gene ID (NCBI): 12490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.