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CD34 Recombinant antibody

CD34 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240659C7

Cat no : 83713-2-RR

Synonyms

CD34 antigen, 240659C7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD34 Recombinant antibody (83713-2-RR)

    WB analysis using 83713-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83713-2-RR (CD34 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CD34 Recombinant antibody (83713-2-RR)

    IHC staining of mouse testis using 83713-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83713-2-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CD34 Recombinant antibody (83713-2-RR)

    IF Staining of NIH/3T3 using 83713-2-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD34 antibody (83713-2-RR, Clone: 240659C7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83713-2-RR

    Affinity and Kinetic Characterization of 83713-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83713-2-RR against Mouse CD34 were performed. The affinity constant is 1.09 nM.

Tested Applications

Positive WB detected inC2C12 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83713-2-RR targets CD34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD34 antigen
Calculated Molecular Weight41 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberNM_133654
Gene Symbol CD34
Gene ID (NCBI) 12490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
WB protocol for CD34 antibody 83713-2-RRDownload protocol
IHC protocol for CD34 antibody 83713-2-RRDownload protocol
IF protocol for CD34 antibody 83713-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols