Biotin Anti-Human CD314/NKG2D (1D11) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-65643

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD314/NKG2D (1D11) Mouse IgG2a RecAb (Biotin-65643, Clone:1D11) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL647-65559, Clone: UCHT4). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65643

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD314/NKG2D (1D11) Mouse IgG2a RecAb (Biotin-65643, Clone:1D11) and CoraLite®488-conjugated streptavidin (red) or unstained (blue). Cells were not fixed.

CD314/NKG2D {ptg:Brand} Recombinant Antibody for FC

Cat No. Biotin-65643

Clone No.1D11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

KLRK1, CD314, 1D11, KLR, Killer cell lectin-like receptor subfamily K member 1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65643 targets CD314/NKG2D in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: NKL cell line
• Full Name: killer cell lectin-like receptor subfamily K, member 1
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC039836
• Gene Symbol: KLRK1
• Gene ID (NCBI): 22914
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).