CoraLite® Plus 405 Anti-Human CD314/NKG2D (1D11)

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  FC experiment of human PBMCs using CL405-65188

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD8 and 5 ul CoraLite® Plus 405 Anti-Human CD314/NKG2D (CL405-65188, Clone:1D11) or CoraLite® Plus 405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65188

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD314/NKG2D (CL405-65188, Clone:1D11) or CoraLite® Plus 405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD314/NKG2D Monoclonal Antibody for FC

Cat No. CL405-65188

Clone No.1D11

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65188 targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NKL cell line
• Full Name: killer cell lectin-like receptor subfamily K, member 1
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC039836
• Gene Symbol: KLRK1
• Gene ID (NCBI): 22914
• RRID: AB_3672391
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P26718
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).