CoraLite®594-conjugated CD31 Monoclonal antibody

CD31 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

3F8E2

Cat no : CL594-66065

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

• 

  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

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  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

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  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in yellow) and Phalloidin (in green). DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

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  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in teal) and Phalloidin (in orange). DAPI in gray. Image credit: Shuntaro Yamada, PhD,

• 

  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in blue) and Phalloidin (in green). DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

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  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in purple) and Phalloidin (in teal). DAPI in yellow. Image credit: Shuntaro Yamada, PhD,

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue, human placenta tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL594-66065 targets CD31 in WB, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD31 fusion protein Ag1787
• Full Name: platelet/endothelial cell adhesion molecule
• Calculated Molecular Weight: 83 kDa
• GenBank Accession Number: BC022512
• Gene Symbol: CD31
• Gene ID (NCBI): 5175
• RRID: AB_2883500
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
human	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang