CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3F8E2

Cat no : 66065-2-Ig

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker



Tested Applications

Positive WB detected inhuman peripheral blood platelets, Jurkat cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue, human liver cancer tissue
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66065-2-Ig targets CD31 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag19730
Full Name platelet/endothelial cell adhesion molecule
Calculated Molecular Weight 83 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC022512
Gene Symbol PECAM1
Gene ID (NCBI) 5175
RRIDAB_2918476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 66065-2-IgDownload protocol
IHC protocol for CD31 antibody 66065-2-IgDownload protocol
IF protocol for CD31 antibody 66065-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

J Neuroinflammation

Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization

Authors - Tianyi Shen
IF

JCI Insight

Uric acid formation is driven by crosstalk between skeletal muscle and other cell types

Authors - Spencer G Miller
IF

Phytother Res

Carnosol alleviates sepsis-induced pulmonary endothelial barrier dysfunction by targeting nuclear factor erythroid2-related factor 2/sirtuin-3 signaling pathway to attenuate oxidative damage

Authors - Xingbing Li
humanIF

Int Immunopharmacol

Clinical significance of Interleukin 17 receptor E in diabetic nephropathy

Authors - Jingjing Wang
humanWB,IHC

Int J Biol Sci

KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy

Authors - Lihong Lu
humanIF

Pharmaceuticals (Basel)

CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway

Authors - Hai-Zhou Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

  • Applications: Immunofluorescence