CoraLite®594 Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

390

Cat no : CL594-65058

Synonyms

Pecam1, PECAM 1, Pecam, 390

---

Validation Data Gallery

• 

  IF Staining of bEnd.3 using CL594-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite®594 CD31 antibody (CL594-65058, Clone: 390 ) at dilution of 1:200.

• 

  FC experiment of C57BL/6 mouse bone marrow cells using CL594-65058

  1x10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD31 (390) (CL594-65058, Clone: 390) or 0.5 ug CoraLite®594 Rat IgG2a Isotype Control (2A3) (CL594-65209, Clone: 2A3), and 0.5 ug APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

---

Tested Applications

• Positive IF/ICC detected in: bEnd.3 cells
• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• RRID: AB_2919928
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).