CoraLite® Plus 488-conjugated CD31 Recombinant antibody

CD31 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4N13

Cat no : CL488-80530

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

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Validation Data Gallery

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  IF Staining of HUVEC using CL488-80530

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of HUVEC using CL488-80530

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80530 targets CD31 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD31 fusion protein Ag1787
• Full Name: platelet/endothelial cell adhesion molecule
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC022512
• Gene Symbol: CD31
• Gene ID (NCBI): 5175
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).