Anti-Mouse CD31 Rabbit Recombinant Antibody

CD31 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

242041B5

Cat no : 98258-1-RR

Synonyms

PECAM-1, Pecam1, PECAM 1, Pecam, 242041B5

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98258-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit Recombinant Antibody (98258-1-RR, Clone: 242041B5) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98258-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit Recombinant Antibody (98258-1-RR, Clone:242041B5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98258-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98258-1-RR against Mouse CD31 were performed. The affinity constant is 0.547 nM.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98258-1-RR targets CD31 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: platelet/endothelial cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• GenBank Accession Number: NM_001305157.1
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).